Figure 2
From: Plexin B3 promotes neurite outgrowth, interacts homophilically, and interacts with Rin
Detection of plexin B3 protein in human and murine brain. (A) Western blot detection of human plexin B3 (B3) protein in pIRES/B3-tranfected COS-7 cells (lane 1) and human brain (corpus callosum, lane 3; neocortex, lane 4) using antibody pAbB3-B. COS-7 cells transfected with empty pIRES served as negative control (lane 2). (B) Western blot detection of B3 protein in mouse brain (lane 1) and of C-terminally truncated recombinant mouse B3 in pcDNA/mB3V5-transfected COS-7 cells (lane 2) using antibody pAbmB3. COS-7 cells transfected with empty pcDNA3.1D/V5-His served as negative control (lane 3).