Detection of plexin B3 protein in human and murine brain. (A) Western blot detection of human plexin B3 (B3) protein in pIRES/B3-tranfected COS-7 cells (lane 1) and human brain (corpus callosum, lane 3; neocortex, lane 4) using antibody pAbB3-B. COS-7 cells transfected with empty pIRES served as negative control (lane 2). (B) Western blot detection of B3 protein in mouse brain (lane 1) and of C-terminally truncated recombinant mouse B3 in pcDNA/mB3V5-transfected COS-7 cells (lane 2) using antibody pAbmB3. COS-7 cells transfected with empty pcDNA3.1D/V5-His served as negative control (lane 3).