Subcellular localization of Rin and of co-localization of Rin and B3 at the plasma membrane. COS-7 cells were transiently transfected with pIRES/B3 encoding non-tagged full-length plexin B3 and pFLAG/Rin encoding FLAG-tagged full-length human Rin and analyzed by confocal laser scanning microscopy. (a) B3 was labeled using pAbB3-B and an Alexa-Fluor®568 (red)-conjugated secondary antibody. (b) Rin was labeled using anti-Flag and an Alexa-Fluor®488 (green)-conjugated secondary antibody. (c) Overlay of the images shown in a and b; yellow signals represent co-localization of B3 and Rin at the plasma membrane (arrowheads). (d) Magnification of the region marked in c. (e) Phase-contrast image of the cell shown in a-c.