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Figure 13 | BMC Neuroscience

Figure 13

From: Plexin B3 promotes neurite outgrowth, interacts homophilically, and interacts with Rin

Figure 13

Plexin B3 interacts with Rin in the Sos recruitment system. Various pMyr and pSos plasmid combinations (as indicated on the left) were used for co-transformation of cdc25H yeast cells representing a positive control (line 1) and negative controls (lines 2–7, 10). Six independent transformants were spotted and grown for six days on glucose medium at 22°C (left panel) or 37°C (middle panel), and on galactose medium at 37°C (right panel). Cdc25H cells re-transformed with pSos-B3IC and pMyr-Rin (line 8) grow on galactose medium at 37°C for six days (right panel), showing interaction between full length human Rin and the intracellular part of human B3 in this system. Cells re-transformed with pSos-B2IC and pMyr-Rin (line 9) show no growth on galactose medium at 37°C after six days (right panel), demonstrating that the intracellular part of human B2 and human Rin do not interact in this system. Rin co-transfected with pSos-vector without insert is not able to induce growth on galactose at 37°C (line 10).

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