Figure 10

Plexins B2 and B3 promote Ca²⁺/Mg²⁺-dependent cell aggregation. Cells stably expressing the neuronal cell adhesion molecule L1 known to promote Ca²⁺-independent cell aggregation were used as positive control. Time-dependent decrease of aggregation index N_t/N₀ indicates decreasing total number of particles due to an increasing proportion of cells aggregated. Given are mean values of three independent experiments with error bars = S.E. of mean × 1. Aggregation assays were performed in DMEM (A), HBSS without Ca²⁺/Mg²⁺ (B), and in HBSS with 1 mM Ca²⁺/0,5 mM Mg²⁺ (C).