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Figure 1 | BMC Neuroscience

Figure 1

From: Evidence for proteolytic cleavage of brevican by the ADAMTSs in the dentate gyrus after excitotoxic lesion of the mouse entorhinal cortex

Figure 1

Schematic representation of the micro-dissection of dentate gyrus and entorhinal cortex: This procedure was developed to isolate dentate gyrus and entorhinal cortex tissue from different rostral-caudal slabs in fresh-frozen mouse brain. A: Coronal section of mouse brain showing bore holes left from blunt-ended, 22 gauge needle punch (green) in the ipsilateral and contralateral dentate gyrus. B: Coronal section of mouse brain (2 mm caudal from section in "A") showing bore holes left from blunt-ended, 18 gauge needle punch (green) in the ipsilateral and contralateral entorhinal cortex. C: Sagittal view of 2 mm slices used for the collection of dentate gyrus (A) and entorhinal cortex (B). Diagrams adapted from 'The Mouse Brain' [46].

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