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Figure 2 | BMC Neuroscience

Figure 2

From: Dynamin-dependent NMDAR endocytosis during LTD and its dependence on synaptic state

Figure 2

NMDAR-mediated responses are not depressed by LFS in recently unsilenced synapses. A. An individual experiment showing a silent synaptic connection with no AMPAR-mediated transmission at -65 mV. Depolarization of the postsynaptic cell to +40 mV revealed the NMDAR-mediated responses (left arrow). Following the induction of LTP by pairing (downwards arrow; [6]), AMPAR-mediated responses appeared (at -65 mV). LFS was performed 10 minutes after the synapse was potentiated. LFS caused no depression of AMPAR-mediated transmission. Subsequent application of the AMPAR antagonist NBQX (10 μM) and depolarization of the postsynaptic cell to +40 mV revealed the NMDAR-only EPSC (right arrow), which was then compared with the NMDAR EPSC before synapse unsilencing (left arrow). Insets (1–5) show example traces at -65 mV (1) and +40 mV (2) prior to pairing; at -65 mV post-pairing (3), at -65 mV post-LFS (4) and at +40 mV during NBQX application (5). B. Data from six paired recording experiments, each performed as shown in part (A) showing that NMDAR-mediated transmission was unchanged by LFS in recently unsilenced synapses. Offset symbols show the average of the six experiments.

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