Figure 1

Altering lin-12 activity increases spontaneous reversal rates. Animals of the genotypes indicated were tested for mean number of reversals per 3 minutes on NGM agar plates (see Methods for details). lin-12(lf) is lin-12(n941), a complete loss of function allele. lin-12(gfcs) is lin-12(n137n460), a cold sensitive, gain of function allele. lin-12p::lin-12(+) and lin-12p::lin-12(OE) are transgenic animals that have been injected (at different concentrations, see Methods) with plin-12::gfp, a plasmid that expresses a functional lin-12 cDNA fused to gfp under the control of the lin-12 promoter 37. *** p<0.0001 vs. wild type.