Figure 1

Example phenotypes identified in the screen. A: NMJs on embryonic ventral longitudinal muscles 7, 6, and 13, visualized using anti-HRP antibodies (which stain all neuronal membrane) and anti-GluRIIA antibodies (which stain postsynaptic glutamate receptors). HRP immunoreactivity is red; GluRIIA immunoreactivity is green. In wildtype NMJs (A, left column), GluRIIA immunoreactivity appears as puncta, representing clusters of postsynaptic glutamate receptors. In homozygous P{SUPor-P}KG00333 mutants (A, right column), GluRIIA immunoreactivity is eliminated. B: NMJs on third instar larval longitudinal muscles 7 and 6, visualized using anti-HRP and anti-GluRIIA antibodies. In wildtype NMJs (B. left column), GluRIIA immunoreactivity appears as large blobs, representing developmentally merged puncta, at sites of innervation. In homozygous P{SUPor-P}Chro [KG03258] mutants (B, middle column), synaptic GluRIIA immunoreactivity is dramatically decreased, although extrasynaptic receptors remain prominent. In contrast, synaptic GluRIIA immunoreactivity is dramatically increased in homozygous P{SUPor-P}vri [KG01220] mutants (B, right column). Scale bars: 10 micrometers.