Movements of BDNF-GFP puncta in an axon and dendrites of a living neuron. A, Image of a cortical neuron which expressed BDNF-GFP in soma, axon and dendrites. Time-lapse images in each square along an axon and a dendrite are magnified and shown in B and C, respectively. Scale bar, 50 μm. B, Moving (b and c) and non-moving (a) puncta in the axon. Each image was taken at a time point indicated at the top of each figure. Right is the distal side of the axon. Scale bar in the bottom image indicates 5 μm and applies to all the images in B and C. C, Moving (a and b) and non-moving (c) puncta in the dendrite. Right is the distal side of the dendrite. Each image was taken at a time point indicated at the top of each figure.