Figure 2

Simultaneous expression of BDNF-CFP and NGF-YFP in the same neuron and proportion of neurons with granular expression of fluorescent puncta. A, Image of a neuron expressing BDNF-CFP. Scale bar indicates 50 μm and applies to B and C. B, Image of the same neuron as in A, expressing NGF-YFP. C, Superimposed images of A and B. D, Proportion of neurons with granular expression of fluorescent puncta. Numerators at the right end of bar indicate the number of neurons showing granular expression of each neurotrophin tagged with CFP or YFP, and denominators indicate the total number of cells which expressed both fluorescent signals. E, Mean proportions of neurons with granular expression of fluorescent puncta, obtained from all the data including those obtained with the single injection of plasmid cDNAs. Numbers at the right end of bar indicate the number of injection trials, in which 4–10 neurons expressed fluorescent signals. Horizontal lines at the end of bars indicate 2SEMs.