Q-PCR validation of AP-1 induction and microarray results. A) Quantitative comparisons of transcript levels in adult head RNA from RU486 treated (AP-1 induced) versus control (black) and control-control (gray) samples. Values represent average cycle difference in PCR product between samples being compared (N = 5). Positive values indicate an increase in transcript compared to unchanging reference gene, negative values a decrease. fos, jun, white and CG6044 RNA levels are increased in the fly head after AP-1 induction in the nervous system while untreated control levels show no significant difference. B) Comparisons of transcript levels in adult head RNA from RU486 treated versus control samples. CG2016, CG11191 and CG15438 levels are induced in all samples from flies fed the steroid hormone RU486 (N = 3). X-axis in panel B indicates the UAS-transgene(s) induced by ElavGS-GAL4 (AP1: UAS-fos;UAS-jun). *Average difference between samples significant at P < 0.05 (Student's t-test).