Acute induction of hepact in larval neurons. A) RNA in situ hybridization using a probe specific for hep shows inducible expression of hep mRNA in third instar larval CNS. B) Western blot analysis of larval CNS protein extracts shows increased levels of activated JNK (P-JNK) after hepact induction. C) A lacZ enhancer trap line of puc shows increased lacZ expression after hepact induction in larval CNS. D) A distribution of up- and down- regulated SAGE tags comparing hepact induced and control libraries, indicates most tags are present in similar numbers in both the induced and control libraries. E) Functional classification of SAGE tags upregulated after hepact induction. Approximately 50% of tags map to genes with no known function and to regions of the genome without an identified gene.