Figure 6
Effect of time and concentration of glutamate on [Ca2+] i . Neuronal cells were maintained for 7 days in conditions similar to those described under Figure 1. Cells were loaded with 5 μM Fluo-3 AM and treated with various concentrations of glutamate. Changes in fluorescence were monitored using the Fluorskan Ascent FL and results were converted to changes in [Ca2+]i (nM) as described in the materials and methods. A. [Ca2+]i measurements were made every 15 secs. Baseline levels of [Ca2+]i were monitored for 60 secs prior to injection with various concentrations of glutamate (indicated by glu). Following injection changes in [Ca2+]i were monitored for an additional 120 secs. B. Initial peak [Ca2+]i change measured at 15 secs post glutamate injection. Each point/bar represents a mean of 8 replicates. Error bars represent SEM.