Figure 10

Detection of NSE (green) and GFAP (red) by immunocytochemistry in neurons and glia. Cells were labelled with either anti-NSE or anti-GFAP and visualized by fluorescence microscopy. Neuronal cells were grown in Neurobasal medium supplemented with 2% B27 and maintained for 7 days in culture. Glial cells were grown in DMEM supplemented with 10% Fetal Bovine Serum and maintained for 7 days in culture. (A) Neurons labelled with anti-NSE. (B) Glial cells labelled with anti-GFAP. Images were taken at 300× magnification.