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Table 1 Autoradiographic analysis of GABAA receptor binding sites in horizontal sections of wild type and mutant mice.

From: GABAAreceptor γ2 subunit knockdown mice have enhanced anxiety-like behavior but unaltered hypnotic response to benzodiazepines

BRAIN REGION

Ligand/condition

Ctx

Th

Hi

CPu

IC

Gr

Mol

[ 3 H]Ro15-4513, basal

+/+ mice

102 ± 3

53 ± 3

102 ± 1

52 ± 4

63 ± 7

93 ± 4

84 ± 4

+/F mice

97 ± 2

42 ± 2c

91 ± 3c

44 ± 3b

52 ± 7

74 ± 7b

72 ± 7a

F/F mice

77 ± 6c

23 ± 2c

68 ± 5c

30 ± 3c

38 ± 13b

59 ± 7a

62 ± 6c

[ 3 H]Ro15-4513 + Diazepam 100 μM

+/+ mice

4.4 ± 0.3

4.0 ± 0.3

7.0 ± 0.4

3.7 ± 0.3

3.3 ± 0.2

56 ± 5

5.4 ± 1.0

+/F mice

3.8 ± 0.3a

3.5 ± 0.4

5.7 ± 0.5c

3.3 ± 0.3

3.3 ± 0.3

41 ± 5c

4.7 ± 0.9

F/F mice

3.1 ± 0.2c

2.8 ± 0.2c

4.3 ± 0.3c

2.8 ± 0.2c

2.9 ± 0.1a

13 ± 4c

3.7 ± 1.5

[ 3 H]Muscimol, basal

+/+ mice

16 ± 2

20 ± 4

9.3 ± 1.3

11 ± 2

4.2 ± 0.6

94 ± 7

8.7 ± 2.3

+/F mice

18 ± 2

23 ± 3

10.0 ± 2.1

11 ± 2

3.9 ± 0.8

101 ± 8

8.6 ± 1.0

F/F mice

15 ± 3

23 ± 7

9.7 ± 3.0

11 ± 4

3.8 ± 0.6

89 ± 16

8.1 ± 3.8

[ 35 S]TBPS, basal

+/+ mice

210 ± 26

583 ± 143

211 ± 30

257 ± 55

914 ± 41

641 ± 114

80 ± 25

+/F mice

271 ± 47

527 ± 110

248 ± 34

277 ± 31

866 ± 51

722 ± 140

73 ± 16

F/F mice

277 ± 57

460 ± 145

299 ± 43b

292 ± 50

829 ± 98

425 ± 32b

100 ± 30

[ 35 S]TBPS + GABA 1 mM

+/+ mice

3.0 ± 2.0

14 ± 9

1.7 ± 1.8

0.4 ± 2.6

4.6 ± 2.2

67 ± 6

1.7 ± 1.8

+/F mice

7.0 ± 1.3a

29 ± 13

5.9 ± 1.6b

4.9 ± 0.4a

18 ± 5a

75 ± 12

0.7 ± 2.4

F/F mice

16 ± 2c

42 ± 16a

13 ± 2.0c

18 ± 3c

76 ± 12c

86 ± 13a

5.0 ± 2.2

  1. The data are means ± standard deviations for 5 mice in each genotype, and are expressed as nCi/mg for 3H-ligands and as nCi/g for 35S-ligand. Significance of the difference from wild type (Tukey-Kramer test after ANOVA): aP < 0.05, bP < 0.01, cP < 0.001. Ctx, cortex; Th, thalamus; Hi, hippocampus; CPu, caudate putamen; IC, inferior colliculus; Gr, cerebellar granule cell layer; Mol, cerebellar molecular layer.
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BMC Neuroscience

ISSN: 1471-2202

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