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Figure 1 | BMC Neuroscience

Figure 1

From: Transcriptome analysis in primary neural stem cells using a tag cDNA amplification method

Figure 1

Experimental approach; A) Schematic overview of the utilised amplification protocol. For details see text. B) Experimental design. NSCs were isolated from the lateral ventricular region of brains from three pools of mice (three isolations) and grown as neurospheres. Sample G was induced to differentiate by withdrawing the growth factors from the culture medium, plating on solid support and adding serum. RNA was isolated from different passages as indicated and used for subsequent microarray hybridisations. Blue arrows represent duplicate hybridisations, arrowhead represents labelling with Cy5 and arrow tail represents labelling with Cy3.

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