Figure 3

Differential distribution of Cln8 mRNA in brains of P0, P5, P10 and adult mouse. Dark-field emulsion autoradiographs from frontal sections, shown on the left, were analyzed by in situ hybridization analysis (A-D). Higher magnification in the hippocampal area CA3 is shown on the right. Bright-field views of the cells (a-d) and dark-field emulsion autoradiographs (a'-d') are shown. Optical density values of hybridization signals of Cln8 antisense probe are shown as bars in the diagram below (A = adult). Optical density values of hybridization signals of Cln8 sense probe are shown as a gray area behind the bars. In each brain region the optical density values at P0, P5 and P10 were compared to the optical density value of adult. Error bars represent standard error of the mean. Symbols *, ** and *** represent p < 0.05, 0.01 and 0.001, respectively. CX = cortex, DG = dentate gyrus