Double immunohistochemical staining of BrdU+ cell clusters in the striatum. In lesioned animals, from 3 days post lesion until 7 days post lesion grouped BrdU+ cells were found in the parenchyma a short distance away from the ventricular wall. Double labeling immunofluorescence studies followed by confocal imaging revealed that striatal BrdU+ cells colocalized with GFAP (arrow, a, b-c) and nestin (d, e-g). Within these groups it was possible to located cells with unipolar (arrow, a), bipolar (arrows, b), and typical mature astrocyte star-like morphology (b, c). Few BrdU+ cells were seen to colocalize with TL (h). BrdU (red); nestin, TL, GFAP (green).