Phenotypes of proliferating cells in the SVZ. Optical microscope studies of coronal brain sections after double-labeled immunofluorescence showed that there is a characteristic placement of cell phenotypes along the entire length of the ventricular wall (vw). Photographs show TL+ ventricular ependymal cells (arrow, a) bordered BrdU+/nestin+ cells (arrows, b), which were found next to GFAP+ cells (arrow, c). Photographs of confocal imaging in the SVZ (more specifically, in zone a of the SVZ) revealed a similar placement where TL+ ependymal cells (d) of the ventricular wall are seen next to BrdU+/nestin+ progenitor cells (e; arrow, f) and GFAP+ cells (g). BrdU+/nestin+ cells outlined in white in photograph e can be seen at greater magnification in f. This patterning was seen at all time points except at postnatal day 9 (P9). At this time, in both control (P9) and lesioned brains (10 h), BrdU+/GFAP+ cells were also seen next to the ventricular wall (asterisk, h). At 10 hours post lesion, a decrease in the number of GFAP+ filaments in the ipsilateral (ip; i) hemisphere was also noted when compared to contralateral (cl; h) and control hemispheres. BrdU (red); nestin, TL, GFAP (green).