Figure 4

Immunohistochemical staining for BrdU in the SVZ, RMS and SGZ. The significant decrease in BrdU+ cell number in the ipsilateral (ip) SVZ following NMDA-induced excitotoxicity is shown in a coronal brain section at 3 days post lesion (3d; compare a, b). BrdU+ cells at all time points in the ip SVZ showed a sharp decrease in number when compared to control hemispheres until 40 days post lesion (40d), the last time point studied, where no difference was seen (c, d). In the ip RMS, a decrease in BrdU+ cells was seen at 10 hours post lesion (10 h) and at 3 days post lesion (e) when compared to the corresponding control RMS (P9 and P12 brains, respectively; f). Thereafter, the ip RMS (7–40 days post lesion) when compared to controls (P16-P49, respectively), showed no difference in BrdU+ cell numbers (g, h). The dentate gyrus of control and NMDA-injected hemispheres showed no differences in cell number at any time point, as shown in a coronal section of the DG at 3 days post lesion (i, j). Note the hippocampal shrinkage in the ipsilateral hemisphere (j).