Figure 9

Flattened growth cones and processes show co-localization of tubulin and Hsp27. This figure shows another example of a neuron treated with the p38 MAPK inhibitors as outlined in Figure 8. Colocalization of Hsp27 (B, red) with tubulin (A, green) is apparent in the emerging processes (C, arrows), and in the flattened and splayed growth cones (A-C, arrowheads); scale bar – 50 μm. At a higher magnification (D-F), loss of microtubule bundling is observed (arrowheads) along with the fibrillar nature of Hsp27 and colocalization with tubulin (arrows); scale bar – 20 μm.