Figure 7

p38 MAPK inhibition blocks phosphorylation of Hsp27. Neurons plated on laminin were exposed to p38 MAPK inhibitors, SB203580 and SB202190 (10 mM each). Cells were sampled at 24 hrs post SB addition, using cellular subfractionation (as described in the Methods). The resulting protein from cytosol, membrane, nucleus and cytoskeleton fractions was electrophoresed and the blot subsequently probed for pHsp27 and Hsp27. Inhibition of p38 MAPK activity (laminin+SB) results in attenuation of the Hsp27 phosphorylation.