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Figure 6 | BMC Neuroscience

Figure 6

From: Hsp27 and axonal growth in adult sensory neurons in vitro

Figure 6

Disruption of the actin cytoskeleton results in aberrant neurite growth. Neurons plated on LN were treated with cytochalasin D (2 mM, added 3 hrs after plating), fixed 24 hrs later and stained for pHsp27 (B, H), Hsp27 (E, K), actin (A, D) or tubulin (G, J). The respective merged images are presented in panels C, F, I and L. The cytochalasin D treatment resulted in various atypical patterns of growth. One phenotype was the elaboration of numerous processes or microspikes as seen in panels A-C, with obvious accumulation of actin and pHsp27 especially at the tips of the microspikes (arrowheads); pHsp27, but not actin, accumulates in the nucleus (B, C, arrow). Abnormal process extension was also observed. In the neuron shown in D-F, some extension was observed although there was now less colocalization of actin with the Hsp27 (arrowheads). Panels G-L show tubulin staining along with either pHsp27 or Hsp27; the nature of the cytoskeletal network is clearer in these examples (arrows). Arrowheads point to atypical neurite growth, eg., lacking the usual radial branching pattern as seen in Fig 4. Scale bar – 20 mm.

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