Figure 5

Co-localization of Hsp27 and actin in growth cones of growing neurites. Growth cones from neurons plated on laminin as outlined for Figure 4 were observed to express both pHsp27 and Hsp27. pHsp27 (A) and Hsp27 (C, E, red), shown together with tubulin (green-yellow) in the merged images (B, D, F), are present in growth cones and filopodia extending from the growth cones (arrows). There is also an accumulation in the core of growth cones and at points of neurite branching (arrowheads- A-F). Note that the tubulin staining does not completely overlap with pHsp27 or Hsp27, particularly in some of the extending filopodia (B, arrow) and the core of the growth cones in D, F (arrowheads). Scale bar – 10 mm.