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Figure 3 | BMC Neuroscience

Figure 3

From: Hsp27 and axonal growth in adult sensory neurons in vitro

Figure 3

pHsp27 also co-localizes with actin and tubulin at early stages of neurite growth. Neurons plated on polylysine and stimulated with laminin for 1–6 hrs were also immunostained with antibodies directed against phosphorylated Hsp27 (pHsp27Ser15) to examine colocalization with actin or tubulin. A, D – rhodamine-phalloidin (red); B, E, H, K – pHsp27 (green); G, J – tubulin (red). The respective merged images are presented in panels C, F, I, L. Actin and pHsp27 appear to be colocalized in the body of the lamellopodium in A-C, but actin seems to be excluded from the leading edge (arrows). There is also localization of pHsp27 and actin in focal contacts (D-F, arrows). pHsp27 also colocalizes with tubulin in focal contacts (G-I, arrow), in a cortical ring (arrowhead) and in processes emerging from the cell body (J-L, arrow). Scale bar – 20 μm.

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