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Table 1 The effect of (1DMe)NPYF on the specific binding of Tb-DeltI on CHO/MYChDOR cells. The KD and BMAX values for Tb-DeltI binding were determined in the presence of different concentrations of (1DMe)NPYF. Native DeltI (1 μM) was used to determine the nonspecific binding. Statistical significances were calculated relative to specific binding in the absence of (1DMe)NPYF (one-way Anova, ***p < 0.001, n = 3). The values in the table are from whole cell assays and therefore provide only relative information about the KD and BMAX values between treatments.

From: Functional modulation of human delta opioid receptor by neuropeptide FF

(1DMe)NPYF (nM) KD(nM) BMAX(pmol/cell)*
0 75.8 ± 8.1 1.60 ± 0.06
1 52.1 ± 6.5 0.70 ± 0.08 ***
10 55.5 ± 7.6 0.89 ± 0.06 ***
100 82.5 ± 3.9 1.42 ± 0.11
1000 80.5 ± 8.2 1.49 ± 0.10
  1. *Relative BMAX-values from whole cell binding experiments; calculated as pmol/cell.