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Figure 3 | BMC Neuroscience

Figure 3

From: The role of the cytoskeleton in cell body enlargement, increased nuclear eccentricity and chromatolysis in axotomized spinal motor neurons

Figure 3

Electron microscopic appearance of the spinal motor neuron cytoskeleton. Embedment-free electron micrographs of two motor neurons within frog lumbar spinal cord extracted as described in Methods. The extracted spinal cord, from which the motor neurons were not isolated, was fixed with 2.5% glutaraldehyde and embedded and sectioned in DGD. After removal of the embedment medium and repeated drying through the critical point, the sections were placed on Formvar- coated grids without staining and visualized by transmission electron microscopy. Nu = nuclear skeleton; nl = nuclear lamina; cy = cytoplasmic skeleton. Inset bar = 10 μm; at higher magnification, bar = 250 nm.

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