Hippocampal injury following hypoxic-ischemic injury in a P17 mouse. A – HI injury (small arrows), triple filter 100X; B – colocalization with NeuN and BrdU, triple filter, 400X; C – BrdU label alone, Cy3 filter, 400X; D – NeuN label alone, FITC filter, 400X; and E – Hoechst counterstain, DAPI filter, 400X. B through E shows the same field. The large arrows show a neuron with BrdU and NeuN staining, demonstrating that the cell has recently divided. The circles show the three areas where the counting was performed.