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Figure 2 | BMC Neuroscience

Figure 2

From: Glutamate-induced apoptosis in primary cortical neurons is inhibited by equine estrogens via down-regulation of caspase-3 and prevention of mitochondrial cytochrome c release

Figure 2

Detection of glutamate induced DNA fragmentation (DNA ladder) Primary cortical cells isolated from 18–20 day old fetal rat brains were cultured for 7 days and treated with 1 mM glutamate for 18 h. The cells were processed as described under "Methods". The isolated DNA was electrophoresed on TBE agarose gel for 1.5 h at 100 V. The DNA fragments were visualized by staining with ethidium bromide. Lane 1 = 1 kb DNA ladder; lane 2 = DNA from untreated cortical cells prior to culture; lane 3 = DNA from untreated control cells cultured for 1 day; lane 4 = DNA from untreated control cells cultured for 2 days; lane 5 = DNA from cells cultured for 2 days and treated with glutamate for 18 h; lane 6 = DNA from untreated control cells cultured for 7 days; lane 7 = DNA from cells cultured for 7 days and then treated for 18 h with glutamate. Note the potentiation of DNA fragmentation in presence of glutamate.

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