Effects of various concentration of glutamate on Fas receptor protein levels in primary cortical cells. Cortical cells isolated from 19 day old fetal rat brains were cultured for 7 days and treated with 1,5,10 and 20 mM glutamate for 6 h. Cells were harvested and total protein was extracted. Fas receptor protein levels were assessed by Western blot analysis as described under Figure 6. The results show that glutamate had no significant effect on Fas receptors. Data are the mean (± SEM) from 3 experiments.