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Table 1 Percentage of ST111/111 cells showing cytoplasmic/nuclear huntingtin localization comparable to wild-type ST7/7 cells in response to chemical treatment*

From: Reversal of a full-length mutant huntingtin neuronal cell phenotype by chemical inhibitors of polyglutamine-mediated aggregation

Compounds Concentration (μM)
  0.5 5 10 50
Gossypol-acetic acid 0 4.5 ± 4.3% 3.5 ± 3.5% 15.9 ± 11.1%
Gambogic acid 1 ± 2.6% 0 2.1 ± 4.6% Toxic (100%)
Juglone 10.4 ± 12.4% 37.9 ± 19.9% 68.8 ± 18.4% N.A.
Celastrol 8.7 ± 8.7% 69.2 ± 3.8% (1%) 78.1 ± 3.5% (4%) N.A.
Sanguinarine 0 (4.3%) 3.7 ± 5.2% (5.5%) 0 (22.5%) N.A.
Anthralin 4.6 ± 7.6% 6.6 ± 9.7% 9.6 ± 12.1% 11.8 ± 14.4%
  1. ST111/111 cells with both nuclear and cytoplasmic staining were counted, as well as total cells and dead cells. The effectiveness of the drugs was measured by percentage of cells with nuclear and cytoplasmic staining versus total cells. In untreated cultures, 89% of ST7/7 striatal cells show both nuclear and cytoplasmic immunostaining, while only 1% of ST111/111 striatal cells show this pattern. Entries in the table represent the average values from 5–10 different microcope fields ± standard deviation. Numbers in brackets indicate percentage of dead cells in cases where toxicity was observed.