Localization of huntingtin in wild type and mutant striatal cell from knock-in mice. Cells were cultured for 48 hours and immunostaining was done with a specific antibody against huntingtin, AP229, followed by Cy3 labeled secondary antibody. The signal was visualized by confocal microscopy (magnification: ×20). A. The localization of full-length huntingtin of striatal cell lines: ST7/7 and ST111/111 with 2.5% DMSO. B. The localization of huntingtin of mutant striatal cell, ST111/111, after treated with drugs at 10 μM.