Dose-response curve of mutant huntingtin aggregation inhibitors. Compounds were added in 1 μl DMSO to 40 μl 20 μg/ml (0.25 μM) GST-Q58-Htn pre-incubated with thrombin. The reaction was carried out at room temperature for 24 hours. The intensity of each dot of the dot blot was normalized to the DMSO control. Assays were performed twice, in triplicate at each concentration each time (n = 6).