PI3K activity mediates leptin activation of K
Representative cell-attached recordings with leptin (10 nM), present in the recording electrode. A, traces illustrate that leptin-induced increase in KATP activity is not reversed on bath application of the MEK inhibitor, PD 98059 (50 μM). The corresponding diary plot for part of the recording, initiated 20 mins after recording began, and following attainment of maximal leptin-induced KATP channel activity is shown. B, traces show that leptin-induced KATP channel activity is inhibited by subsequent application of the PI3K inhibitors, wortmannin (10 nM) or LY294002 (10 μM). Corresponding diary plots for Nf.Po from a single experiment for each PI3K inhibitor are shown below the relevant traces.