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Figure 2 | BMC Neuroscience

Figure 2

From: Foxo3a induces motoneuron death through the Fas pathway in cooperation with JNK

Figure 2

Subcellular localization of Foxo3a reflects its state of phosphorylation by Akt. (A, B): Motoneurons were electroporated with constructs encoding HA-tagged version of wildtype Foxo3a (wt Foxo3a; A) or the constitutively active triple-mutant form (TM Foxo3a; B), together with another vector encoding GFP. They were cultured for 24 hr either in the presence of NTFs, or in their absence. In the latter case, they were treated with the PI3K inhibitor LY 294002 (100 μM) for the last 90 min before fixation. HA-wt-Foxo3a and HA-TM-Foxo3a were detected staining for the HA tag. Characteristic labelled cells are illustrated for each condition, together with nuclear labelling using DAPI. Scale bar = 5microns. (C) Quantification of subcellular localization in A and B. Approximately 20 motoneurons were analysed in each condition in duplicate wells; results of two independent experiments are shown as mean ± range.

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