Figure 4
Colocalization of the MT 1 receptor with phenotypic markers in C17.2 NSCs. Cells were maintained in 10% FBS + 5% HS for 9 days and then in 1% FBS for 3 days before seeding on coverslips in 1% FBS for 2 days. (A,B,C) Confocal images of MT1 (green), Nestin (red), and MT1 & nestin. (D,E,F) MT1 (green), GFAP (red) and MT1 & GFAP. (G,H,I) MT1 (green), β-tubulin III (red) and MT1 & β-tubulin III. Double-labeled cells exhibit yellow-orange fluorescence. Scale bars: A-F: 50 μm; G-I: 20 μm.