SLOB expression is altered in Clkjrkand Pdf01flies. (A) Brain wholemount from wild type rosy (ry) and Clkjrkflies. Two time points, taken on the second day of DD, show that SLOB does not cycle in the PI neurons of the Clkjrkflies. The accompanying bar graph illustrates relative intensity scores. Data were analyzed as described in Figure 5. (B) Brain wholemount from wild type y w and Pdf01flies. Two time points, taken on the second day of DD, demonstrate the decrease in SLOB expression and dampened cycling in the PI neurons of Pdf01flies. PDF appears to positively regulate SLOB protein. The bar graph represents relative intensity scores.