SLOB cycling is altered under DD conditions in clock mutants. (A) Western blot analysis of SLOB cycling in Canton S, per01, tim01and Clkjrkflies under LD conditions. Oscillations in all flies are in phase with one another. The graphs represent the mean ± SEM for each time point from a minimum of three experiments for each genotype. (B, C) Western blot analysis of Canton S, Clkjrkand per01flies in DD conditions. There is no obvious cycling of SLOB in Clkjrkand per01flies. (D) Western blot analysis of Canton S and tim01flies in DD conditions. There is a shift in the phase of SLOB cycling in tim01flies. The peak is no longer at CT 14 but at CT 2. The same Canton S data are illustrated in panels (B-D ).