Figure 5

Immunohistochemistry of catfish barbel taste buds using antibodies GP1 and GP2. Barbels were fixed in 4% PFA, PBS, and cryostat sectioned at 10 microns. Sections were probed using indicated dilutions of antibodies GP1 and GP2. (A). Section through a barbel immuno-stained with GP1 at 1/8000 dilution. The lines from "TB" point to labelled taste buds. (B). The apical aspects of three taste buds immuno-stained with 1/12000 dilution of GP2. (C). Cross section through a taste bud immuno-stained with 1/16000 dilution of GP1. (D). A single taste bud immuno-stained with 1/8000 dilution of GP2. (E). Second antibody control showing barbel without exposure to primary antibodies.