Figure 3

SDS-PAGE (4–20%) of solubilized barbel protein, visualized with silver stain, before (A) and after (B, C, D) each enrichment step. Each lane was loaded with 5 μg of protein. Lane A: Total protein of fraction Sp. Lane B: Galactose eluted protein from the RCA column containing L-Arg stimulated ion channel activity; Lane C: Protein of the first peak fractions of the Sephacryl gel filtration step containing L-Arg stimulated ion channel activity; and Lane D: Protein containing L-Arg stimulated ion channel activity from the ion exchange chromatography enrichment step.