Concentration
| Pigment Index± SEM |
n
|
---|
0 nM | 0.74 ± 0.01 | 4 |
1 nM | 0.77 ± 0.03 | 4 |
10 nM | 0.80 ± 0.02 | 4 |
100 nM | 0.78 ± 0.02 | 4 |
1 μM | 0.78 ± 0.01 | 4 |
- RPE was isolated from dark-adapted bluegill and, following incubation for 45 minutes in 10 μM forskolin, was incubated an additional 45 minutes in increasing concentrations of arecaidine but-2-ynyl ester tosylate or in a DMSO control. At the end of incubation in forskolin, RPE had a pigment index of 0.72 ± 0.02 (n = 4 fish); the 0.01% DMSO control had a pigment index of 0.69 ± 0.02 (n = 2 fish).