Figure 1From: Lithium facilitates apoptotic signaling induced by activation of the Fas death domain-containing receptorLithium promotes apoptotic signaling mediated by Fas in Jurkat cells. Jurkat cells were pretreated with 20 mM lithium for 30 min as indicated, followed by treatment with an agonistic anti-Fas antibody (5, 10, 20, or 50 ng/ml). After 24 hr, immunoblots were used to detect (A) active caspase-3, and (B) cleaved PARP. Densitometry was used to measure immunoreactive bands and the ratios of the responses in the presence and absence of lithium were calculated (shown under the immunoblots; Mean ± SEM; n = 3).Back to article page