SAP47 expression in the Sap47 cDNA RNAi lines. (A). Western blot of head extracts (one head/lane) from control (WT: wild type (w1118)) and five independent RNAi transgenic lines (VII.5, VI.1, V, I.13, I.61). SAP47 protein levels in VII.5 and V RNAi transgenic lines is drastically reduced. CSP: loading control. (B). Semi-quantitative Western blot of head extracts from Sap47 cDNA RNAi expressing flies (line VII.10) and wild type as control. WT: wild type (w1118). x-values indicate the number of heads loaded per lane. Actin-Gal4/VII.10: animals expressing RNAi under the control of actin-Gal4. RNAi reduces SAP47 protein levels in these flies by an approximate factor of 16. Normal SAP47 expression is observed in RNAi VII.10 (RNAi line without a Gal4 driver) and in actin-Gal4 (driver line alone). CSP: loading control.