Figure 3

SAP47 expression in the Sap47 mutants generated by P-element jump-out. (A). Western blot analysis of head extracts from jump-out lines (Sap⁴ – Sap²⁰⁸) and wild type (WT) control. The jump-out lines Sap⁴, Sap²⁶, and Sap⁷⁰ show a reduction in SAP47 expression, the lines Sap¹⁵⁶, Sap²⁰¹, and Sap²⁰⁸ lack any detectable SAP47 expression. CSP: loading control. (B). Immunohistochemical staining of frozen head sections. WT: wild type control; Sap¹⁵⁶: Sap47 jump-out null mutant line.