SAP47 expression in the Sap47 mutants generated by P-element jump-out. (A). Western blot analysis of head extracts from jump-out lines (Sap4 – Sap208) and wild type (WT) control. The jump-out lines Sap4, Sap26, and Sap70 show a reduction in SAP47 expression, the lines Sap156, Sap201, and Sap208 lack any detectable SAP47 expression. CSP: loading control. (B). Immunohistochemical staining of frozen head sections. WT: wild type control; Sap156: Sap47 jump-out null mutant line.