A and B show the operated facial nucleus 3 dpo. Neither in bright field (A) nor in dark field (B) a specific in situ hybridization signal could be observed with the sense probe. C and D: In sham operated animals no SCD-1 up-regulation was observed using antisense probes. E shows the negative control of the immunohistochemistry, no signal could be detected by omitting the primary antibody. Scale bars represent: 50 µm.