SCD-1 specific radioactive in situ hybridization of facial nucleus neurons 3 and 7 dpo. A and B show the operated side 3 dpo; numerous strongly labeled cells (arrows) are visible in dark filed microscopy (A) which were identified as neurons by bright field microscopy of the same area (B, arrows). High power magnification of axotomized facial nucleus neurons 7 dpo (C and D) verifies the neuron-specific SCD-1 expression due to their large diameter and characteristic thionine staining. No SCD-1 mRNA induction could be identified in non-axotomized neurons (arrows) of the control side (E and F). Scale bars represent: 200 µm (A, B, E and F), 50 µm (C and D).