Isoform specific transcriptional regulation of Ant genes by TGF-β1. A. Schematic diagram of Ant2 promoter and Ant1+Ant2 chimeric constructs used in transcription reporter assays. Ant2 5.6 kb construct contains Ant2 promoter sequence (gray bar) upstream of the transcription start site (TSS; black arrow). Ant2 2.2 kb contains promoter sequence proximal to the TSS and some downstream sequence (red bar). The Ant1+Ant2 Chimera construct was generated by inserting the 88 bp Ant1 promoter sequence of Ant1Prom3-5' (white bar) upstream of Ant2 2.2 kb. B. TRAs with primary astrocytes transiently transfected with Ant2 2.2 kb. Reporter firefly luciferase activity (normalized to Renilla luciferase activity) is expressed as the mean ± SEM with the number of replicates performed for individual treatment conditions shown in each bar. p ≤ 0.05 for two-tailed student's t-test. C. TRAs with primary astrocytes transiently transfected with Ant1+Ant2 Chimera. p = 0.017 for one-tailed student's t-test.