Figure 1

TGF-β1 regulation of Ant1 in primary astrocytes. A. Schematic diagram of Ant1 promoter full-length and deletion reporter constructs used in transcription reporter assays. B. Primary astrocytes were transiently transfected with the indicated reporter constructs. Reporter firefly luciferase activity (normalized to Renilla luciferase activity) is expressed as the mean ± SEM with the number of replicates performed for individual treatment conditions shown in each bar. The average TGF-β1 induction of each construct was compared to untreated controls transfected with the same construct by student's t-test. Asterisks denote p ≤ 0.05. C. Immunoblot analysis of Ant1 protein in control untreated (U) and TGF-β1 treated (T) astrocyte cultures. Immunoblots were subsequently stripped and reprobed with an antibody to p42/44 MAPK to demonstrate the amount of protein loaded in each lane.