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Figure 3 | BMC Neuroscience

Figure 3

From: Identification of regeneration-associated genes after central and peripheral nerve injury in the adult rat

Figure 3

Differentially expressed genes within red and Clarke's nuclei. Non-radioactive ISH illustrates the differential expression of clone U41 seven days after spinal cord hemisection. A: Dramatic up-regulation of mRNA of clone U41 within axotomized Clarke's nucleus neurons (small arrows) ipsilateral and caudal to the lesion. Clarke's nucleus neurons of the control, non-operated side (large arrow) show only very faint expression. Bar, 100 μm B: High magnification of axotomized Clarke's nucleus neurons (arrows) demonstrates increased mRNA expression of clone U41. Bar, 20 μm C: Negative control for ISH using a sense probe. Bar, 100 μm D: Low magnification of the midbrain at the level of red nucleus. Up-regulation of clone U41 has been detected within axotomized red nucleus neurons, contalateral to the lesion (OP). Red nucleus neurons of the control side (CON) show only faint expression. Bar, 400 μm E: High magnification of red nucleus contralateral to the lesion reveals increased mRNA expression of clone U41 within axotomized neurons. Bar, 100 μm F: Red nucleus neurons of the control side showing weak mRNA expression. Bar, 100 μm

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