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Table 2 Secondary antibodies and probes used for immunofluorescence and western blot analysis.

From: Discrete nuclear structures in actively growing neuroblastoma cells are revealed by antibodies raised against phosphorylated neurofilament proteins

Antibody/Probe (Catalog #) Conjugate Dilution Supplier
Goat anti-mouse IgG (#A-11001) Alexa-fluor 488 1:200 in PBS Molecular Probes, Eugene, OR
Goat anti-rabbit IgG (#T-6778) Alexa-fluor 488 1:200 in PBS Molecular Probes, Eugene, OR
Goat anti-mouse polyvalent (#A-0162) Alkaline Phosphatase 1:4000 in PBS Sigma Chem. Co, St. Louis, MO
Goat anti-rabbit IgG (#A-3812) Alkaline Phosphatase 1:30,000 in PBS Sigma Chem. Co., St. Louis, MO
Nucleic Acid binding probe; TO-PRO-3 iodine (#T-3605) --- 1:1000 in PBS Molecular Probes, Eugene, OR