Table 2 Secondary antibodies and probes used for immunofluorescence and western blot analysis.
Antibody/Probe (Catalog #) | Conjugate | Dilution | Supplier |
|---|---|---|---|
Goat anti-mouse IgG (#A-11001) | Alexa-fluor 488 | 1:200 in PBS | Molecular Probes, Eugene, OR |
Goat anti-rabbit IgG (#T-6778) | Alexa-fluor 488 | 1:200 in PBS | Molecular Probes, Eugene, OR |
Goat anti-mouse polyvalent (#A-0162) | Alkaline Phosphatase | 1:4000 in PBS | Sigma Chem. Co, St. Louis, MO |
Goat anti-rabbit IgG (#A-3812) | Alkaline Phosphatase | 1:30,000 in PBS | Sigma Chem. Co., St. Louis, MO |
Nucleic Acid binding probe; TO-PRO-3 iodine (#T-3605) | --- | 1:1000 in PBS | Molecular Probes, Eugene, OR |